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KMID : 0900920030270020179
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Korean journal of Animal Reproduction
2003 Volume.27 No. 2 p.179 ~ p.185
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Analysis of Transgenic Mouse, for the Production of Immunodeficiency Animals
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Naruse Kenji
Lee Seung-Hyun
Choi Hwa-Sik
Lee Seung-Ho
Park Chang-Sik
Jin Dong-Il
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Abstract
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To determine whether the diphtheria toxin-A (DT) gene disrupts development of thymocytes in transgenic animal, the DT-A gene was used for the production of transgenic mice directed by proximal Ick promoter sequences. Two transgenic founder mice that contained several copies of transgene were produced by DNA microinjection and integration of transgene in transgenic mice was confirmed by PCR and Southern blotting analysis. Transgenic F_1 and F_2 mice were produced by outbreeding of founder and F_1 mice to investigate expression of transgene and phenotypes in transgneic mice. Expression of the diphtheria toxin gene was confirmed in thymus, spleen and liver of transgenic mice by RT-PCR. In circulating blood of transgenic mice, lower number of circulating white blood cells and platelets were observed compared with that of normal mice. In addition, transgneic mice had reduced number of circulating peripheral T-cells analyzed by FACS with anti-CD3 antibody. The data in these transgenic mice indicate that DT gene can play a disruptive role in developing thymocytes of transgenic mice resulted in lower number of T-cells that can be applicable to a wide range of tissues in other animals.
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KEYWORD
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Diphtheria toxin-A, lck promoter, Transgenic mice, T-cells
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